Program of Differential Gene Expression Leads to the Different Cell Types in a Multicellular Organism

Rucete ✏ Campbell Biology In a Nutshell

Unit 3 GENETICS — Concept 18.4 A Program of Differential Gene Expression Leads to the Different Cell Types in a Multicellular Organism

Multicellular organisms develop from a single fertilized egg through a highly orchestrated process of differential gene expression. Cells become specialized, forming distinct tissues and organs through regulated gene expression during embryonic development.

Genetic Program for Embryonic Development

  • Cell division: increases cell number.
  • Cell differentiation: cells specialize in structure and function.
  • Morphogenesis: arrangement and organization of cells into specific structures.

Cytoplasmic Determinants and Inductive Signals

  • Early embryonic cells receive developmental instructions from:
    • Cytoplasmic determinants:
      • Substances (RNA, proteins) unevenly distributed in the egg cytoplasm by the mother.
      • After fertilization and division, these determinants lead cells down different developmental pathways by regulating gene expression.
    • Inductive signals:
      • External signals from neighboring embryonic cells.
      • Cells influence one another's developmental paths via signaling molecules and surface receptors, activating gene expression pathways.

Sequential Regulation of Gene Expression in Differentiation

  • Determination: when embryonic cells irreversibly commit to a specific cell fate. Initially subtle, detectable through gene expression.
  • Differentiation: actual physical and functional specialization of cells, marked by production of tissue-specific proteins.
  • Muscle cell differentiation example:
    • Early embryonic cells become myoblasts, committed precursors to muscle cells.
    • MyoD gene encodes MyoD protein, a master regulator that triggers other muscle-specific genes and halts cell division.
    • Result: formation of mature skeletal muscle fibers from fused myoblasts.

Pattern Formation and Axis Establishment

  • Pattern formation: the spatial arrangement of tissues and organs.
  • Controlled by positional information provided by cytoplasmic determinants and inductive signals.
  • Body axes (anterior-posterior, dorsal-ventral, left-right) are established early.
  • Drosophila studies:
    • Homeotic genes direct pattern formation and body plan development.
    • Maternal effect genes (egg-polarity genes) deposit determinants in the egg, setting body axes.
    • bicoid gene: specifies anterior (head) structures; bicoid mRNA and protein form gradients that guide anterior-posterior axis development.

In a Nutshell

Differential gene expression orchestrates the development of multicellular organisms from a single fertilized egg. Cytoplasmic determinants, inductive signals, and sequential gene activation guide embryonic cells through determination, differentiation, and morphogenesis, creating specialized cell types and organized body structures.

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